Bovine ILsize:6 ELISA Kit, 96 test
Bovine INS (Insulin) ELISA Kit: Overview
The Bovine INS (Insulin) ELISA Kit is a highly sensitive and specific assay designed for the quantification of insulin in bovine serum, plasma, and other biological fluids. This immunoassay is based on the enzyme-linked immunosorbent assay (ELISA) method, which allows for precise measurement of insulin levels in various biological samples. It is commonly used in research and veterinary diagnostics to study insulin secretion and regulation in bovine species, which can be vital for understanding metabolic disorders such as diabetes and insulin resistance in cattle.
Key Features
- Target Analyte: Insulin (INS) from bovine sources.
- Assay Type: Sandwich ELISA, which uses a pair of antibodies to capture and detect the insulin antigen in the sample.
- Detection Range: The kit provides a wide dynamic range, capable of detecting low and high concentrations of insulin with high accuracy.
- Sample Types: Suitable for bovine serum, plasma, and other related biological fluids.
- Detection Method: Colorimetric detection via a substrate reaction, with results read using a microplate reader.
- Sensitivity: The kit is designed to detect even small changes in insulin concentrations, ensuring precise measurements for research and diagnostic purposes.
Principles of the Assay
The Bovine INS ELISA Kit utilizes the principle of a sandwich immunoassay, where the insulin molecule is "sandwiched" between two specific antibodies. One antibody is immobilized on the surface of a microplate, while the second antibody, which is linked to an enzyme, binds to the captured insulin. Following the binding steps, a substrate is added, which reacts with the enzyme, resulting in a color change. The intensity of the color produced is directly proportional to the concentration of insulin in the sample, allowing for quantification.
Components of the Kit
- Coated Microplate: Pre-coated with capture antibodies specific to bovine insulin.
- Standards and Controls: Known concentrations of bovine insulin used to generate a standard curve for quantification.
- Detection Antibody: An enzyme-conjugated secondary antibody that binds to the captured insulin.
- Substrate Solution: Reacts with the enzyme to produce a measurable color change.
- Washing Buffer and Sample Diluent: Used for washing and preparing samples for the assay.
Procedure
- Sample Preparation: Serum or plasma samples are diluted to the appropriate concentration using the provided sample diluent.
- Assay Setup: The diluted samples and standards are added to the wells of the pre-coated microplate.
- Binding: Insulin present in the samples binds to the capture antibody immobilized on the plate.
- Detection: The detection antibody is added, which binds to the captured insulin, forming a complex.
- Color Development: After adding the substrate, the enzyme reacts to produce a color change.
- Measurement: The absorbance is measured using a microplate reader, with results interpreted based on the standard curve.
Applications
- Veterinary Diagnostics: Used to assess insulin levels in bovine health, aiding in the diagnosis of metabolic disorders, including insulin resistance and diabetes.
- Endocrinology Research: Essential for studying bovine metabolic responses, including insulin secretion in response to various stimuli.
- Production Studies: Helps in monitoring insulin regulation in livestock, which can affect growth and production efficiency.
Advantages
- High Sensitivity: Capable of detecting low levels of insulin with high precision.
- Wide Detection Range: Ideal for both clinical and research applications involving bovine insulin.
- Quick and Easy Protocol: Requires minimal preparation and provides results within hours.
- Reproducibility: Offers consistent and reproducible results for reliable analysis.
Conclusion
The Bovine INS ELISA Kit is an invaluable tool for accurately quantifying insulin in bovine biological samples. It is designed to meet the needs of both clinical diagnostics and research applications, offering reliable and reproducible results that help further the understanding of bovine metabolic processes.
